TCA protein precipitation protocol

Stock Solutions: 100% (w/v) Trichloroacetic acid (TCA) recipe: dissolve 500g TCA (as shipped) into 350 ml dH 2 O, store at RT. (for details, check Maniatias under TCA ppt ) Precipitation Protocol: 1. Add 1 volume of TCA stock to 4 volumes of protein sample. i.e. in 1.5ml tube with maximum vol., add 250µl TCA to 1.0ml sample. 2.

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